Remove nucleic acids from and reduce viscosity of protein extracts with Benzonase® Nuclease, a genetically engineered endonuclease from Serratia marcescens. It eliminates all forms of DNA and RNA (single-stranded, double-stranded, circular or linear) more efficiently than DNase I without affecting proteins. The enzyme is functional over a wide range of conditions and possesses an exceptionally high specific activity. Adding rLysozyme™ Solution, a highly purified recombinant lysozyme, which degrades bacterial cell walls, enhances extraction efficiency, especially for larger proteins. rLysozyme™ Solution exhibits 250 times higher specific activity than chicken egg white lysozyme.
Features & Benefits
- Compliant with FDA guidelines for nucleic acid contamination Increase protein extraction efficiency and facilitate downstream processing of protein extracts with the combined activities of rLysozyme™ Solution and Benzonase® Nuclease
- Effectively reduce viscosity and remove nucleic acids from protein solutions
- Effective over a wide range of conditions and has an exceptionally high specific activity
- Functional between pH 6 and 10, from 0 °C to 42 °C for maximum versatility
- Active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea
- Convenient: Available in two purity grades (ultrapure >99% and pure >90%) and in two concentrations (standard at 25 U/?L and high concentration, HC, 250 U/?L)
- Versatile: Compatible with a variety of lysis reagents such as BugBuster® and CytoBuster™ reagents to eliminate viscosity and increase protein yields
- Total endotoxin: < 0.25 EU/1,000 units.
- Purity: > 99% by SDS-PAGE
- Concentration: 25 U/ul
- Specific Activity: 1 x 10e6 units/mg protein
- Store at -20 degrees