Amicon® Ultra-2 centrifugal filter devices for protein or DNA purification and concentration provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex samples . When used for DNA purification and concentration the Amicon® Ultra 0.5 device provides the best balance between PCR recovery and PCR primer removal for double stranded DNA base pairs form 137 to 1159. The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarisation and subsequent contamination of the membrane are also minimised by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample is achieved by a convenient reverse spin step after collecting the filtrate. The device can be spun in a swinging bucket or fixed angle rotor.

Advantages:

• Sample concentration from 2 mL down to 15-70 µl
• 98% sample recovery
• Engineered dead stop
• Fast processing time of 10 – 30 minutes
• Reverse spin capability provides consistent recoveries

Applications:

• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
• Purification of macromolecular components found in tissue culture extracts or cell lysates
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
• Desalting, buffer exchange, and protein dialysis
• Size exclusion and protein fractionation

Specifications:

• Maximum initial sample volume – 2 mL
• Typical final concentration volume – 15 – 70 µl
• Optimal and maximum relative centrifugal force – Swinging bucket rotor 4,000 x g for concentration spin 1,000 x g for recovery spin
• Optimal and maximum relative centrifugal force – Fixed angle rotor 7,500 x g for concentration spin 1,000 x g for recovery spin
• Active membrane area - 1 cm²
• Hold up volume – < 5 µl