Overview

Amicon® Ultra-15 centrifugal filter devices for protein purification and concentration provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex samples . The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarisation and subsequent contamination of the membrane are also minimised by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipettor, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor.

Advantages:

High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
Vertical membrane reduces concentration polarisation for ultra-fast spin times (as fast as 10-15 minutes)
Heat-sealed membrane minimises downstream extractables
High retentate recovery of >90%
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume graduations
Direct pipette sample access eliminates processing step to recover concentrate
High concentration factors of 80-100X

Applications:

Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange, and protein dialysis
Size exclusion and protein fractionation

Specifications:

Maximum initial sample volume – 15 mL (35? angle fixed rotor is 12 mL)
Typical final concentration volume – 200 µl
Centrifugal Force Swinging bucket rotor: 4,000 x g; Fixed angle rotor (35?): 5,000 x g
Active membrane area – 7.6 cm²
Diameter – 2.97 cm