MILUFC900308 - Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-3 membrane, 3 KDa 15mL /PK 8
Amicon® Ultra-15 centrifugal filter devices for protein purification and concentration provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex samples . The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent contamination of the membrane are also minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipettor, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor.
Advantages:
- High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes)
- Heat-sealed membrane minimizes downstream extractables
- High retentate recovery of >90%
- 100% integrity tested for reliable performance
- Convenient sample monitoring with translucent housing and volume graduations
- Direct pipette sample access eliminates processing step to recover concentrate
- High concentration factors of 80-100X
Applications:
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting, buffer exchange, and protein dialysis
- Size exclusion and protein fractionation
Specifications:
- Maximum initial sample volume – 15 mL (35 angle fixed rotor is 12 mL)
- Typical final concentration volume – 200 µl
- Centrifugal Force Swinging bucket rotor: 4,000 x g Fixed angle rotor (35): 5,000 x g
- Active membrane area – 7.6 cm² • Diameter – 2.97 cm