Amicon® Ultra-15 centrifugal filter devices for protein purification and concentration provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex samples . The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent contamination of the membrane are also minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipettor, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. Advantages: • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes) • Heat-sealed membrane minimizes downstream extractables • High retentate recovery of >90% • 100% integrity tested for reliable performance • Convenient sample monitoring with translucent housing and volume graduations • Direct pipette sample access eliminates processing step to recover concentrate • High concentration factors of 80-100X Applications: • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC • Concentration of dilute or pre-purified proteins from column eluents • Desalting, buffer exchange, and protein dialysis • Size exclusion and protein fractionation Specifications: • Maximum initial sample volume – 15 mL (35? angle fixed rotor is 12 mL) • Typical final concentration volume – 200 µl • Centrifugal Force Swinging bucket rotor: 4,000 x g Fixed angle rotor (35?): 5,000 x g • Active membrane area – 7.6 cm² • Diameter – 2.97 cm