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  • Guanidine thiocyanate for molecular biology, =99%, 250g (Class 8 pkg III UN 1759)

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Guanidine thiocyanate for molecular biology, =99%, 250g (Class 8 pkg III UN 1759)

Product Code: SIGG9277-250G

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Overview

Application

Guanidine thiocyanate has been used:
  • - for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
  • - as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
  • - for DNA extraction from cerebrospinal fluid (CSF)
Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples.Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Technical Specifications
Supplier Merck

PROPERTIES

Biological source

Synthetic (organic)

Quality Level

200

Grade

for molecular biology

Assay

≥99%

Form

Powder or crystals

Technique(s)

RNA extraction: suitable

Colour

Colourless to white,

MP

120-122 °C (lit.)

Solubility

Water: 100 mg/mL, clear, colorless

Cation traces

Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

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