Heat-activatable form of KOD DNA Polymerase for increased specificity and convenient PCR setup

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KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3??5? exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits. Source: Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli Concentration: 1.0 U/µl Nicking activity: None detected Amplification efficiency: Functional PCR; inhibition of activity at 21°C verified Storage: –20°C

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